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KMID : 0900920010250020093
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Korean journal of Animal Reproduction
2001 Volume.25 No. 2 p.93 ~ p.100
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Effect of Estrus Cycle on the Neclear Development of Preantral Follicle Oocytes in Canine
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Cho Sung-Kyun
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Abstract
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This study was conducted to evaluate the nuclear development of preantral follicle oocytes in dog following collecting from different estrus ovaries and oocyte diameter. To do this, ovaries were collected from Sunchon livestock station by ovarioectomy and then transported to laboratory in 30^{circ}C saline within 2 h. All of the ovaries were washed three times with saline supplemented with 100 IU Penicillin and 100 mutextrm{g}/mell Streptomycin and then sliced with blade in 100 mm dish. All of the oocytes collected were classified the oocyte size such as over 110 {mu}{textrm}{m} or under 110 {mu}{textrm}{m} and the estrus status. To induce the nuclear development, oocytes were cultured in TCM199 alpha-MEM supplemented with 10% FCS, 0.1 mutextrm{g}/mell sodium pyruvate, 100 ng/mell FSH, 100 ng/mell EGF, 1% ITS, 100 IU/mell penicillin, 100 mutextrm{g}/mell streptomycin at 38.5^{circ}C, 5% CO_2 incubator for 72 h. After culture, all of the oocytes were stained in 1% orcein following fixed in 45% Acetic acid for 48 h. The oocyte recovery rates of over 110 {mu}{textrm}{m} from estrus ovary (63.6%) were significantly higher rather than that in anestrus status (51.5%). Oocyte recovery rate per ovary of over 110 {mu}{textrm}{m} in estrus status ovary (22.6/63.8%) was also significantly higher rather than that in anestrus status ovary (8.2/51.6%). Nuclear development to MI of over 110 {mu}{textrm}{m} in estrus ovary (24.3%) was significantly higher rather than those in under 110 {mu}{textrm}{m} or over and under 110 {mu}{textrm}{m} in anestrus (2.5, 6.8 and 0.0%), respectively (P ＜ 0.05). Nuclear development to AT and MII of over 110 {mu}{textrm}{m} in estrus was more developed in other groups. Nuclear development to MI in TCM199 (21.8%) was significantly higher than in alpha-MEM (10.0%). Altho mutextrm{g}h the development rate to AT was significantly different between TCM199 (7.3%) and alpha-MEM (1.1%), but to MII was not different between TCM199 (0.9%) and alpha-MEM (1.1%). The results indicated that over 110 {mu}{textrm}{m} oocytes was could be recovery from estrus status ovary, bigger oocytes were more developed to MI, AT or MII in TCM199.
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KEYWORD
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Dog, Oocyte, Nuclear development, In vitro maturation
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